Coding

Part:BBa_M33330:Design

Designed by: Frank Li   Group: Stanford BIOE44 - S010   (2010-06-01)

RC of atzR CDS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 34
    Illegal AgeI site found at 196
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We made 4 silent point mutations to make this sequence conform to Assembly Standard 10: 3 for EcoRI and 1 for NotI. We also edited to make TAA the stop codon.

The atzR system may not work in E. coli.

Source

From Pseudomonas sp. ADP at http://www.ncbi.nlm.nih.gov/nuccore/13937422, bp 99885..100853. Formerly called orf99.


References